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临床医学教学中已经形成了一套设计简单、操作可行的“生理学”和“药理学”实验教学项目，且已经配备了相应的设备、设施和试剂。近几年的教学改革中，越来越多的院校将两部分实验整合为医学机能实验。刘爱明、张俊芳编著的《医学机能实验(英文版)》中的实验设计参考了 主要医学院校生理学和药理学传统实验项目的设计，编排顺序参考了“生理学”和“药理学”理论课程的授课顺序，符合系统化教学要求；同时，增加部分创新设计实验和能力拓展型实验，为提升留学生培养质量、引导部分学生独立设计和开展探索性科学实验预置了空间，可以满足国外留学生、双语教学学生、医学专业7年制学生“生理学”和“药理学”两门专业课程实验教学的应用要求。

Chapter 1

Basics of Physiological-Pharmacological Experimentation

Unit 1 Objectives and Requirements of Physiological-Pharmacological Experiment

Unit 2 Process of Experiment Data and Production of Experiment Reports

Unit 3 Requirements and Safety Rules in Laboratory

Unit 4 Operation of Biological Data Acquisition System RM-6240

Chapter 2

General Knowledge of Physiological-Pharmacological Experimentation

Unit 1 Fundamental Principle of Physiological-Pharmacological Experi- mentation

Unit 2 Preliminary Tests in Physiological-Pharmacological Experimentation

Unit 3 Statistical Analysis Most Often Used in Scientific Activities

Unit 4 Statistic Analysis Using Excel

Chapter 3

Basic Operations on Experimental Animals

Unit 1 Fundamentals of Animal Experiments

Unit 2 Operations on ExperimentalAnimals

Unit 3 Gender Identification and Labeling the Animals

Unit 4 Narcotics and Animal Narcosis

Unit 5 Sample Collection and Bleeding

Unit 6 Drug Administration for Laboratory Animals

Chapter 4

Physiological-Pharmacological Experiments

Experiment 1 Generation and Conduction of Compound Action Potential

Experiment 2 Actions of Antiepileptic and Anticonvulsants in Mouse Models

Experiment 2-1 Effect of Drugs on Electric Convulsive Shock

Experiment 2-2 Effect of Drugs on Convulsion Induced by Central Stimulants

Experiment 3 Actions of Analgesics in Mouse Models

Experiment 3-l Analgesic Effect Assayed by Hot-Plate Model

Experiment 3-2 Analgesic Effect Assayed Using Chemical-Stimulation Model

Experiment 4 Influence of Stimulus on the Contraction of Skeletal Muscle

Experiment 5 Determination of ABO Blood Group

Experiment 6 Effects of Physical and Chemical Factors on Blood Coagulation

Experiment 7 Recording of Electrocardiogram in Toads

Experiment 8 Effects of Chemical Agents on the Activity of the Isolated Toad Heart

Experiment 9 Regulation of Artery Blood Pressure

Expenment 10 Measurement of Human Blood Pressure

Experiment 11 Toxic Effects of Cardiac Glycosides on Heart

Experiment 12 Effect of Drugs on Isolated Intestinal Smooth Muscles

Experiment 13 Regulation of Respiratory Movements

Experiment 14 Experimental Pulmonary Edema in Rabbits

Experiment 15 Factors and Drugs Influencing Urine Output

Experiment 16 Drug Actions Influenced by Dosage Forms and Delivery Routes

Experiment 17 Determination of 50% Effective Dose of Pentobarbital

Experiment 18 Measurement of Concentration-Time Profile of Metronidazole

Experiment 19 Calculation of the Pharmacokinetic Parameters

Experiment 20 Intoxication and Detoxification of Organophosphate Agents

Experiment 21 Effect of Glucocorticoids on Intraabdominal Capillary Permeability in Mice

Experiment 22 Effect of Anti-inflammatory Agents on Tumescence in Rat Model

Experiment 23 In-Vitro Experiment of AntibacterialActions

Chapter 5

Expanded Experiments

Experiment 1 Extraction of Genomic DNA in Peripheral Blood

Experiment 2 Extraction of mRNA from Liver Tissues

Experiment 3 Assessment of Gene Tranion Level by Real-Time PCR

Experiment 4 Assessment of Protein Expression by Western-Blot

Experiment 5 Isolation and Culture of Mouse Hepatocytes

Appendix

Table 1 Physiological parameters of experimental animals

Table 2 Age, body weight and lifetime of adult experimental animals

Table 3 Formularies of commonly used saline solution

Table 4 Bleeding methods for experimental animals

Table 5 Safe and lethal bleeding volume of experimental animals

Table 6 Scaling factors for cross-species dose calculation

Table 7 Application route and dose of commonly used anaesthetics

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